Studies on L-Glutamate Decarboxylase Activity in E. coli Part III
نویسندگان
چکیده
منابع مشابه
Interaction of L-threo and L-erythro isomers of 3-fluoroglutamate with glutamate decarboxylase from Escherichia coli.
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis of the unstable intermediate enamine. The ...
متن کاملGenetic analysis of glutamate transport and glutamate decarboxylase in Escherichia coli.
The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport recombinants. Two genes determining the ability to grow on glutamate as the s...
متن کاملBrain L-glutamate decarboxylase: purification and subunit structure.
Glutamate decarboxylase (GDCase; L-glutamate-1-carboxy-lyase, EC 4.1.1.15) was purified from whole rat brain approximately equal to 1300-fold to apparent homogeneity with a specific activity of 2.4 units per mg of protein by a combination of column chromatographies on DEAE-cellulose, hydroxylapatite, and gel filtration, and preparative nondenaturing polyacrylamide gel electrophoresis. The purif...
متن کاملPotentiometric and multi-NMR studies of aluminum(III) complex with L-glutamate in acidic aqueous solutions.
Complex formation studies of L-glutamate with aluminum(III) ion were conducted in acidic aqueous solutions (pH 2.0 - 5.5) by means of pH-metric titration and multinuclear (1H, 13C and 27Al) NMR techniques. The following results were obtained: (1) Al could weakly coordinate with Glu to form various mononuclear 1:1 (AlLH2+, AlL+, AlLH(-1)) species and dinuclear 2:1 (Al2L4+) species in acidic aque...
متن کاملBrain Glutamate Decarboxylase
Glutamate decarboxylase (glutamate 1-carboxylyase, EC 4.1.1.15, GAD)* IS the major, rate-limiting enzyme in brain for synthesizing gamma-aminobutyric acid (GABA). Total GAD activity in brain is 10-20 times greater than the observed rate of GABA synthesis (Collins, 1972; Matsui and Deguchi, 1977; Casu and Gale, 1981), indicating that GAD operates at only a fraction of its capacity. Although othe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1972
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.46.191